Serena Williams' participation in the women's doubles is in doubt as she battles to overcome a knee injury.
The 44-year-old played her first singles match in 1,396 days on Tuesday night as she was beaten by Maya Joint on Centre Court.
Williams took Joint, 24 years her junior, to a deciding set but was unable to complete what would have been an extraordinary comeback.
It was announced after the match that Williams was unable to conduct any of her post-match media duties.
Players are mandated to speak to the press after matches, with the Grand Slam rulebook stating that is obligatory unless they are "unable to appear on reasonable grounds (for instance illness or injury)".
That was the case for Williams and it is why she was not fined for failing to attend her press conference.
She suffered a knee issue during her match with Joint, which stretched for almost two and a half hours, and The Times report that the American required lengthy treatment in the locker room on Tuesday night due to swelling on her right knee.
A statement from Williams' team said: “Serena tweaked her knee at the end of the first set and was therefore excused from her media obligations by the Wimbledon and WTA medical teams.
"She left site that night unaided and is doing everything she can to be ready for her doubles match later this week.”
Williams was awarded a wildcard into the doubles draw alongside her sister Venus.
The pair have won six doubles titles at Wimbledon together, the most recent of which came a decade ago.
The sisters are scheduled to face Solana Sierra and Camila Osorio in their opening round on Thursday or Friday.
Williams now faces a race against time to recover and ensure she has at least one more match at Wimbledon this year.
She is not due to practice on site on Wednesday afternoon.