The pop star played Charlene Robinson on the Australian soap for two years in the 1980s and she will make an incredible return alongside Jason Donovan to mark the ending of the show.
Both Kylie and Jason will return to Ramsay Street for an emotional farewell to the soap, which is wrapping this summer after over three decades on screen, much to fans' disappointment.
Kylie - who went on to become a global pop superstar after her stint on the soap - has now opened up about her experience of filming, describing it as "lovely and emotional".
Speaking on Today Extra, she said: "It was lovely and emotional. Driving into Ramsay Street was quite something."
Asked about her reaction to being back on set, she added: "Firstly, where's all that time gone? Secondly, everything looks exactly the same. Thirdly, Scott, Charlene, Jane, Harold - it all just came flooding back."
Whilst she couldn't say much about her final appearance on the show, she did tease there was a "lot of talk about memories".
"There was a lot of talking about memories and going back in time," she shared of the experience.
Executive producer Jason Herbison previously confirmed on the soap's Instagram account that the pair would be back, adding: "Scott and Charlene are the ultimate Neighbours couple and it would not feel right to end the show without them.
"We are thrilled that Jason and Kylie have come home to play a very special part in our series finale. It has been an emotional experience for them, for us and I'm sure it will be for our viewers."
Fans were left emotional by the news, with one writing: "Amazing way to finish the show off, hats off to Kylie & Jason for been part of #CelebratingNeighbours. This makes me happy to see him and @kylieminogue back on the show. Tissues will be at the ready. This is where I first became a huge fan of Jason seeing him on Neighbours on his first episode."
Another added: "ooooo exciting. But I'd rather the show just continues."
Neighbours was axed after Channel 5 pull the plug on the show, with Fremantle Media confirming they were ceasing production of the Aussie soap in June, with the final scenes being aired later in the summer.