Erika Kirk sat next to an empty chair as she appeared on her late husband's podcast, The Charlie Kirk Show, on Friday, 26 September.
The conservative political commentator and Donald Trump ally hosted the show from 2020 until his death on 10 September.
Speaking on Friday's episode, Erika vowed that the show would "go on" after Charlie was shot and killed while speaking at a university in Utah.
Her appearance came after she said she forgives her husband's killer in an emotional tribute at his memorial service, prompting a standing ovation from the crowd.