Lime street station was one of four train stations found to be Covid free after tests were carried out by Imperial College London.
Four of England's busiest train stations were tested, including London Euston, Birmingham New Street and Manchester Piccadilly.
Surfaces regularly touched by passengers, such as ticket machines, were swabbed and sent away for testing.
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The same was done on intercity train services and hour-long air samples were also analysed.
Experiments were carried out in January and again in June with both cases producing encouraging results.
Data released by the Department for Transport statistics shows the use of national rail services in Britain was at around 14% of pre-pandemic levels in January but was back to around half by June.
At the beginning of January, the pandemic in England was at its peak but cases dropped off by more than 50% by the end of the month.
In June, the opposite was true: the month started with 2,539 new cases being reported but by the 30th it had climbed to 21,293, back to levels last seen in January.
However, on both occasions the results were the same, with stations and trains shown to be clean from any infection.
Rob Mole, senior programme manager for Network Rail’s response to the pandemic, said: “Station cleaning teams and train staff have made it their mission to keep passengers safe during the pandemic and this is proof their dedicated approach works.
“We want all passengers to travel in confidence on the railway network and we will keep doing our part by rigorously cleaning trains and stations.
“We ask passengers to do their bit too by wearing face coverings while travelling out of respect for others so we can all stop the spread of Covid-19.”
Covid-19 is known to transmit primarily via airborne particles but can survive on indoor surfaces for more than 24 hours.
The legal requirement to wear a mask in stations and on trains ended on July 19 but customers are still encouraged to do so.
A survey by Yougov released last week revealed mask-wearing has remained fairly consistent among older age groups despite the rule change, but has dropped to 26% among under-24s.
David Green, senior research fellow at Imperial College London, said: “In the same way that a swab is used to take a Covid-19 test in the nose and throat and sent to the lab, we use a filter to collect any virus particles in the air and swabs to collect viruses on surfaces.
“This approach provides a way of quantifying the amount of virus circulating in these public environments and the effect of mitigation strategies like cleaning and wearing face coverings.
“This is part of a wider programme of work with the public transport sector to understand where this virus is most prevalent so that we can return to pre-pandemic activities as safely as possible.”
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