More than 300 people from Greater Manchester organised coaches to drive down and join the march for a People's Vote in London, in which around one million protesters ground the city to a halt.
Manchester for Europe, Stockport for Europe and NHS Together brought down eight coaches with more than 300 people on them, and were joined in London by a further 200 from the Manchester area who took the train.
Protesters at the march demanded the government 'Put it to the People' , with the masses donning EU flags and placards.
Children in prams, and even attendees in their eighties, joined the groups on the coach down the London.
This comes as more than five million people have signed a petition to revoke article 50 and remain in the European Union.
Catherine Moss, Chair of Manchester for Europe, said that she has seen a huge increase in support for a second referendum in the past six months, with a particular acceleration in the last month.
She said: "I think people are becoming more aware of the damage that is being done and more alarmed at how the government is behaving. The government is making a mess of it."
There are many reasons for wanting a second referendum, ranging from deeply personal reasons, to the NHS, the economy, and what Catherine describes as a 'genuine fear' of the future after we leave the EU.
She said: "I think the NHS is a big part, particularly for people who voted leave on the back of the leave campaign that was NHS focused, as they now realise it was all a lie.
"For the people who voted for remain, for many it is personal because we feel European
"The economy is for many another factor, I feel, on behalf of people who voted leave who are going to be most damaged by it.
"People are genuinely scared. People are becoming frightened; frightened for the implications on peace, especially in Ireland, as this could unravel everything.
"They're frightened of the breakdown of society and the sense that we are being pitted against each other."
For members of the group NHS Together, a Manchester-based group which joined the protest, the NHS is the primary issue of concern.
According to Helen Atkinson, a representative of the group, it was created to focus on the issues that really matter rather than getting dragged down with the heated political debate.
She said: "We know the debate has become quite heated. It's helpful to talk about issues that people care about, therefore, like the NHS.
"The NHS was used by the leave campaign to encourage people to vote leave but most officials within the NHS agree Brexit is bad for the NHS."
She added that those who joined the Manchester groups were from all walks of life.
"I gave stickers to children in prams and I was speaking to a guy in his 80s. When we were at the back of the march near the DJs I was dancing to some really good house music with a guy in his 60s," she said.
"Another man had an operation last week but he took the train down and joined the march for as long as he could manage."
Critics of the movement say that to launch a second referendum would be an affront to democracy.
Just this weekend members of the Brexit Protest and Direct Action Group organised a 40mph 'go-slow' drive between Leeds and Liverpool, while Nigel Farage started a March to Leave from Sunderland to London last week.
But for Catherine Moss, and hundreds of thousands of others who joined her on the march, it would be undemocratic to continue with Brexit as it is.
She said: "Nobody voted for the mess, for the crisis, we are in now.
"The majority of issues people voted on aren't being delivered. Within a democracy we have to have the ability for people to change their mind.
"It would be undemocratic to say: 'This isn't what people voted for two years ago, but tough' in the face of all these people marching and signing the petition."
She added: "I am cautiously optimistic that we will get a people's vote - though I'm not going to say it's inevitable, as we know it's going to be tough."
Revoke Article 50 Brexit petition reaches five million signatures
