Over just six days, they will run 174 miles, climbing more than one-and-a-half times the height of Mount Everest and ending at the top of a volcano.
The 250 participants will make their way the Azores Islands ending at the crater of Sete Cidades on the westernmost point of Sao Miguel.
Those taking part in the "Run for Love 3" event will raise money for the Tribe Freedom Foundation (TFF), which raises money for and works to raise awareness of modern slavery and human trafficking.
Tom Stancliffe, who founded the charity, alongside Guy Hacking and Rob Martineau told The Independent that running symbolises freedom to the group.
He added: “It’s all about community. This is a life changing challenge to make a difference in the world.”
All three will take part in the ultra-marathon, the third "Run for Love" event.
The first between Odessa and Dubrovnik took place in 2013 and the second between Sarajevo and London was four years later.
The organisers aim to raise £250,000 for two frontline modern slavery charities: "Ella’s Home", a safe-house based in East London that helps women escaping situations of trafficking and exploitation, and the Sheffield-based "Snowdrop Project", which helps victims of human trafficking access training, counselling and employment.
The Tribe Freedom Foundation ultra-marathon will take place from November 23 until December 1.
