Uber passengers have to wear a face mask from Monday and drivers can refuse to take people if they don't.
In an email sent to customers on Friday titled "No mask, no ride" the company outlined the new rules.
The policy, which comes into force from June 15, is to protect both drivers and customers.
The email states: "From Monday, if you aren't wearing a face mask or covering, your driver has the right to cancel your ride.
"Similarly, you can also cancel your ride if your driver arrives and is not wearing a face covering.
"Finally, anyone who is repeatedly flagged for not wearing a face covering will risk losing access to the app."
It comes after people in Wales were recently advised to wear masks on public transport and where social distancing is difficult.
On June 9 Welsh health minister Vaughan Gething said that people were not being recommended to wear the face coverings outdoors.
In their email Uber said when wearing a face covering or mask passengers and drivers must make sure it covers "your face from the bridge of your nose down to your chin".
The Welsh Government has also published the technical advice on which it based its decision to change the guidelines.
In a statement Mr Gething said: "Washing your hands, avoiding touching your face, and maintaining a two-metre distance from others remain the best way of stopping the spread of the virus.
"On Friday, the World Health Organisation updated their guidance on face coverings, advising that they should be considered in settings where maintaining social distancing is difficult.
"However, to be crystal clear, wearing a face covering does not replace the need for social distancing or washing your hands regularly.
“Further scientific evidence is needed on the benefits to the wider public of wearing face coverings but observational findings so far suggest that homemade or purchased three-layer face coverings might reduce transmission from one person to another if made, worn, handled, and disposed of properly."
The decision came after the announcement that face coverings would be required on public transport in England from June 15.
In England travellers can be fined or refused permission to board buses or trains if they refuse to comply with the order.
