Twitter users reported significant issues with the social media platform and complained of tweets "not loading" on Saturday night.
Users were met with a warning message that read: "Something went wrong. Try reloading," as some tweets appeared not to be loading.
Despite the problems, others said they were still able to post on the platform.
The website Down Detector, which monitors reports of Twitter outages, saw a large spike in people reporting Twitter going down after 6pm on Saturday.
One user tweeted: "Twitter mentions not working. Got to go to the original tweet to see responses. Anyone else got this issue?"
The suspected outage is more trouble for the platform, bought for a reported £37.9billion in October by Tesla billionaire Elon Musk.
Amid the reported difficulties, Musk responded to one tweet that read: "It always seems a little weird to me that people complain about Twitter on Twitter."
The billionaire replied "It's great" accompanied by a laughing emoji.
On Thursday, the website said it had fixed the "trouble" experienced by iOS users.
The company tweeted: "Pardon the interruption! iOS users may have experienced some trouble using Twitter earlier. Things should be back to normal now."
The day before, people on the site complained that they were able to send messages or post tweets for a period of around 30 minutes.
They were met with an error message that read: "You are over the daily limit for sending tweets."
A series of layoffs were seen in the wake of Musk's takeover and departing engineers warned of possible difficulties on the platform.
Around two-thirds of the company's main service engineers are reported to have been cut.
As the complaints continue, Musk has said Twitter users will have to start paying for two-factor authentication on the site.
Charges for text message two-factor authentication will begin on March 20, as the tech mogul says Twitter is being scammed of $60million (£48million) a year by phone companies.
Twitter said it "will no longer permit non-Twitter Blue subscribers to use text messages as a 2FA method."
"At that time, accounts with text message 2FA still enabled will have it disabled."
