Prime Minister Prayut Chan-o-cha has sacked Deputy Agriculture Minister Thamanat Prompow and Deputy Labour Minister Narumon Pinyosinwat.
Capt Thamanat stands accused of manoeuvring the ouster campaign which allegedly involved a number of Palang Pracharath Party (PPRP) politicians and others in the main opposition Pheu Thai Party.
"It's my reason. It's my business," Prayut said about the sackings.
A source in the PPRP said on Thursday the fate of Deputy Prime Minister Prawit Wongsuwon hangs in the balance following the dismissal of the pair who are his close aides.
Meanwhile, a resignation letter signed by Thamanat was circulated online on Thursday.