The bodies of several more swans have been found at Carlingwark Loch in Castle Douglas.
The dead birds were discovered on Tuesday lying in reeds beside the water.
Bird flu had previously claimed the lives of 20 swans at the popular beauty spot.
The RSPB has been tracking what is believed to be the south west’s worst avian flu outbreak in recent years.
Andrew Bielinski, area manager for the Scottish Lowlands and Southern Uplands, said “I believe there have been a number of dead swans at Carlingwark and that bird flu has been recorded there.
“Advice remains don’t pick dead birds up, even though the risk to humans is very low.
“Reports can still be sent to DEFRA, but they may not test anymore birds from the loch since flu had already been recorded.
“I believe the council is organising collection of dead birds from Carlingwark and some other places.”
Mr Bielinski added: “Birds are continuing to die at Mersehead and Caerlaverock.
“RSPB colleagues will be organising carcass collections from our reserve at Mersehead.
“It’s not a great start to the year.”
At least five avian flu outbreaks have hit the east of the region with more than 700 geese removed from the Mersehead site near Southerness.
Anyone finding sick or dead wild birds can report them to the Department for Environment, Food and Rural Affairs on 03459 33 55 77.
