The Queensland Police Service (QPS) is sifting through records to find staff who have not had a COVID-19 vaccination and may face disciplinary action.
The deadline for officers and staff to have at least a first dose of vaccination passed on Monday.
It said a "significant majority of QPS members" have complied with the vaccine mandate but work was underway to identify those who have not.
"The QPS is working through internal records as a priority to identify which members have not been vaccinated and do not have an approved exemption," the QPS said in a statement.
Police Commissioner Katarina Carroll said the mandate was to help maintain a healthy workforce and maintain "adequate policing services".
"As a frontline service agency, we are more exposed to the risk of COVID-19 than most occupations and sadly we have seen police officers die as a result of the virus around the world, including 90 officers in the United Kingdom alone," she said.
Some staff quit over mandate
Deputy Commissioner Steve Gollschewski confirmed a number of QPS staff had resigned over the vaccine mandate.
"We have had some people resign and that numbers changed on a daily basis, but the numbers are low," he said.
"I'm saying a handful per district across the state — we're collating those figures as we speak at the moment — we'll have a clearer view later in the week."
Early last month, Commissioner Carroll directed all police officers and staff to receive their first dose of the COVID-19 vaccine by October 4 and their second by January 24, 2022.
Last week, seven officers were granted a short delay in the requirement to comply with the rule, after challenging the mandate in the Supreme Court.
