The Palestinian Waqf vowed on Tuesday to defy an Israeli court order barring access to the Golden Gate, a side building at the holy al-Aqsa mosque compound in Jerusalem.
There have recently been scuffles between worshippers and police there over the use the Golden Gate, or Bab al-Rahma, closed by Israel since 2003.
Arguing there was no longer any reason for it to remain shut, Palestinian officials reopened the building last month and crowds of worshippers prayed inside despite the Israeli closure, reported AFP.
A Jerusalem court this week gave the Waqf until March 10 to explain why the closure order should be lifted, Israeli watchdog group Ir Amim said in a statement.
"As the Waqf does not formally recognize the Israeli court system, it is unlikely to issue a formal response, in which case the court is expected to approve closure of the building," the NGO said.
"It is anticipated that a forced closure by the police will trigger significant numbers of Palestinians rallying or breaking the closure."
"The decisions of the courts do not apply to the mosque of Al-Aqsa," Sheikh Abdel Azim Salhab, the leader of the Waqf council, said in a video clip published on Tuesday.
"It is our right, religious and contractual, to access the Golden Gate and keep this door open for Muslims to pray," he said.
Salhab and his assistant were briefly detained last week for what police said was violation of an order preventing entry into a prohibited area of the holy site, said AFP.
They were released later the same day but the arrest drew condemnation from Jordan, the custodian of the Haram al-Sharif, in Israeli-annexed east Jerusalem.
Waqf spokesman Firas al-Dibs said that since the latest dispute erupted Israel had arrested nearly 130 Palestinians in Jerusalem, including senior Muslim officials.
It has temporarily barred more than 60 people from the compound, he said.
Access to Golden Gate was closed by an Israeli court order in 2003 during the second Palestinian intifada over alleged militant activity there, police say.
Waqf officials argue that the organization that prompted the ban no longer exists.
The compound is the third-holiest site in Islam and a focus of Palestinian aspirations for statehood.
It is also the location of Judaism's most sacred spot, revered as the site of the two biblical-era Jewish temples.
Jews are allowed to visit but cannot pray there and it is a frequent scene of conflict between the two sides.
Palestinians fear Israel will seek to assert further control over it, while Israel accuses Palestinians of using such claims as a rallying cry to incite violence.
It is in the walled Old City in east Jerusalem, occupied by Israel in the 1967 Six-Day War and later annexed in a move never recognized by the international community.
