Ole Gunnar Solskjaer has added three players to his Europa League squad following the January transfer window.
United have lost four players from the 25 names originally listed in October for Champions League action prior to their elimination.
Tim Fosu-Mensah and Odion Ighalo both left the club last month, while Jesse Lingard and Facundo Pellistri have each gone on loan.
Solskjaer has given a clear indication of the role he expects Amad Diallo to play over the remainder of the season by including him in his new 24-man selection.
Diallo, 18, is yet to make his senior United debut but is expected to be handed his chance in the coming weeks.
United's first leg of their last-32 tie against Real Sociedad on February 18 may provide the perfect opportunity for Solskjaer to throw the winger into first-team action.
Defender Phil Jones has also been brought back into the fold after his recovery from injury, which kept him off the previous submission to UEFA.
The only other change to United's official squad is the inclusion of academy goalkeeper Nathan Bishop.
The departures of Fosu-Mensah and Lingard took United's pool of homegrown talent down to six players - below the minimum requirement of eight.
Academy stars such as Mason Greenwood and Brandon Williams are still eligible for competition without being counted as part of the permitted group of 24, hence their omission from the named squad.
The same would have been the case for Bishop, but it appears he has been officially selected to help meet UEFA's compulsory quota.
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Man Utd's official Europa League squad
Goalkeepers
David De Gea
Dean Henderson
Lee Grant
Nathan Bishop
Defenders
Eric Bailly
Phil Jones
Victor Lindelof
Harry Maguire
Luke Shaw
Alex Telles
Axel Tuanzebe
Aaron Wan-Bissaka
Midfielders
Amad Diallo
Bruno Fernandes
Fred
Daniel James
Juan Mata
Nemanja Matic
Scott McTominay
Paul Pogba
Donny van de Beek
Forwards
Edinson Cavani
Anthony Martial
Marcus Rashford
