Victoria's Opposition Leader Michael O'Brien is set to face a leadership challenge.
AAP understands Brad Battin will make a tilt for the position during a Liberal partyroom meeting before parliament sits on Tuesday morning.
The move follows months of speculation about the performance of Mr O'Brien as opposition leader.
Mr O'Brien took over the leadership of the party from Matthew Guy after Labor's landslide election victory in 2018.
He has faced criticism for failing to land any blows on Premier Daniel Andrews and his government amid the coronavirus pandemic, particularly when it came to the bungled hotel quarantine program that led to the state's second wave.
An Ipsos poll commissioned by Nine News and The Age, published in October, showed only 15 per cent of the 858 Victorians surveyed approved of Mr O'Brien's performance during the pandemic.
The premier's approval rating was at 52 per cent and has since grown.
Liberal MPs told AAP Tuesday's spill has been "triggered" in part by the WA election at the weekend, which reduced the Liberal party to as few as two seats in the lower house.
But a number of MPs have expressed doubt that Mr Battin will secure enough support to win the ballot.
The party's 31 state MPs will first need to vote to bring on a spill motion and declare the leadership vacant. They will then vote on who they prefer as leader.
Mr Battin is a 45-year-old father of two and the member for Gembrook, situated southeast of metropolitan Melbourne.
He holds the shadow portfolios of roads and road safety, crime prevention, victims support and youth justice.
Tuesday marks the first parliamentary sitting day since the premier was hospitalised due to a serious fall.
Deputy Premier James Merlino will remain acting premier for at least six weeks while Mr Andrews recovers.
