Nine people from six different Championship clubs have tested positive for Covid-19 following the latest round of testing.
A total of 1,094 players and staff were tested in a four-day period ending on Tuesday.
Sheffield Wednesday have confirmed one member of their staff has tested positive for coronavirus. Preston also say an 'individual' at the club has returned a positive test.
Anyone who has tested positive will enter self isolation in line with the guidelines provided by the EFL.
In League Two, where only the four clubs in the play-off places are participating, there were zero positive cases from a sample size of 126.
There is currently no testing programme underway in League One.
English Football League clubs will decide how to end the season on Tuesday, with "a number of proposals" up for discussion amid continued uncertainty in League One.
With the Championship aiming for a June 20 restart and League Two having already accepted an early curtailment, the third tier has yet to form any kind of workable agreement.
Voting on a preferred framework for concluding the 2019-20 campaign had originally been scheduled for Monday but, following formal submissions from clubs, has been moved back 24 hours.
Additional reporting from Press Association
