More than 250 people queuing to see Queen needed medical attention in one day

As many as 259 people queueing to see the Queen Elizabeth II lying-in- state needed medical attention on Saturday, as night time temperatures dropped as low as 4C.

The figure was released by St John's Ambulance Service, which, along with the London Ambulance Service, has been providing medical help to those lining up or visiting the area to pay their respects.

The charity said that overall, 403 people in the "ceremonial areas" of London required medical support on Saturday and 19 of these were taken to hospital.

Meanwhile, in the early hours of Sunday morning - from midnight to 7am - 98 people required their services, nine of whom were taken to hospital.

Thousands of volunteers for the charity have come out to help during the national period of mourning.

Wrapped in blankets, members of the public wait in queue earlier today to see the late Queen (AFP via Getty Images)

The organisation is also providing medical cover in Windsor where huge numbers have gathered each day since the Queen died.

In total hundreds of mourners in the crowds have needed help so far.

Around 1,000 St John Ambulance volunteers will be on duty during the state funeral in London and Windsor, including 800 who will be on hand to provide medical and first aid support.

People rest against the wall as they wait in queue during a chilly Saturday night and Sunday morning (Getty Images)

Earlier today the public were told not to set off to queue for the Queen's lying-in-state - with the line set to close imminently.

The Government is monitoring the situation and will provide an official cut-off point for people hoping to join, with public viewing set to close at 6.30am tomorrow.

A woman receives medical attention in Southwark Park after she became unwell waiting in the queue to view the Queen's coffin (Getty Images)

The Department for Culture, Media and Sport (DCMS) Tweeted that at 4pm the estimated current wait time is at least 10 hours and the end of the queue is in Southwark Park.

Wait times consistently over 12 hours have been recorded ever since the body of the late monarch was brought to London.

The DCMS said: "We are monitoring numbers closely. Some people will unfortunately miss out."

People queuing through the night on the South Bank (AFP via Getty Images)

Temperatures have dropped as low as 4C during the night (AFP via Getty Images)

A government source added: "We don’t want people to make long journeys only to find it closed. The public desire to view the lying-in-state has been phenomenal.

"We will give people as much notice as possible so they can avoid disappointment."

The official cut-off time for people hoping to see the coffin of the monarch is 6.30am on Monday morning.

"We have always said we will need to close the queue well in advance of the lying-in-state ending."