Marcus Rashford took a knee and raised a fist in solidarity with the Black Lives Matter movement ahead of Manchester United's match against Tottenham.
The England striker was in action for the first time since forcing the government into a U-turn over free school meal vouchers for vulnerable children over the summer holidays.
Players from both sides took a knee before the game in London on Friday night.
All four games of the Premier League's 'Project Restart' have been preceded by players and staff performing the gesture, while 'Black Lives Matter' will replace names on the back of shirts for all matches this weekend.
The game has thrown its support behind the movement following the death of George Floyd in the US.
During the warm-up, Rashford and his teammates all wore black t-shirt with 'United against racism' emblazoned on the front.
On Wednesday night, Rashford's England team-mate Raheem Sterling was one of the players to take a knee at the Etihad, before Manchester City played Arsenal.
After the game, he said: "I see it as a massive step for the Premier League to allow something to happen.
"It shows we're going in the right direction. Little by little we're seeing change, and I think that's what black players and most of the rest of the country are hoping for."
In an emotional letter earlier this weekr, Rashford called on MPs to reverse the government's decision to end free school meals for low-income familes.
The England striker said the letter was 'written from the heart' and pleaded for authorities to let vulnerable children to continue receiving the free school means during summer.
After Boris Johnson announced he would be extending the programme Rashford tweeted: "I don’t even know what to say. Just look at what we can do when we come together, THIS is England in 2020."
