Manchester United star Jadon Sancho has now been sent home from the England camp after failing to shake off an injury picked up earlier this week.
The winger was ruled out of Thursday's win over Hungary due to a minor knock, and will now play no part in the fixtures against Andorra on Sunday or Poland on Wednesday.
A statement on the England website read: "Following further assessment, the 21-year-old has returned to his club and is expected to recover in time for Manchester United ’s fixture with Newcastle United on Saturday 11 September. "
Sancho becomes the second player to drop out of Gareth Southgate's squad after Everton striker Dominic Calvert-Lewin withdrew with injury prior to the Three Lions meeting up for duty.
Southgate will now stick with his 23-man squad for the next two World Cup qualifiers without calling up any replacements.
England still top their group after the 4-0 victory in Budapest maintained their 100% record in qualification so far.
But the gloss was soon taken off the win by abhorrent incidences of racist abuse aimed at England players Raheem Sterling and Jude Bellingham.
Sterling opened the scoring but was pelted with missiles from the home crowd inside the Ferenc Puskas Stadium, who threw objects including cups at the forward.
There have also been reports of monkey chants being directed at Sterling and youngster Bellingham, who was one of the substitutes warming up on the touchline.
Hungary have a history of recent racist abuse and were only handed a ban by UEFA to play their next three fixtures behind closed doors after the behaviour of their supporters at Euro 2020.
Thursday's match was allowed to go ahead with a crowd present as World Cup qualifiers come under FIFA rule.
England's players had already been booed when they took the knee prior to kick-off in protest at racism and discrimination, and Southgate has now called on the authorities to take action against Hungary.
“There’s no more this group of players or staff can do in the fight against racism,” England’s manager said. “Other people have got to take the right action to try and make progress.
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“Our players can’t do anything more than they have done in the past two or three years in trying to get the right messages out, take the right stands, and it’s for other people to protect them. It’s for me to protect them in the main, but for the authorities to protect them as well.
“They shouldn’t have to be subjected to any form of racism. There’s a balance in the crowd. As we know at home not everybody causes problems. Tonight our anthem was really respected remarkably well.
“It’s not fair to criticise all the Hungarian fans. A lot were very generous and behaved extremely well. The individuals that are responsible need to be dealt with. I think there’s some evidence that people have been filmed and we’ve got to hope the authorities deal with that in the right way.”
