Sunderland suffered their first League One defeat of the season as Portsmouth ran out 3-1 winners at the Stadium of Light.
The result gives their League One rivals the chance to steal a march of Phil Parkinson's side, although it's still at a very early stage.
Lincoln returned to the top of the table after a 1-0 win over fellow high-flyers Ipswich.
Jorge Grant saw his deflected shot come back off the post and Tomas Holy's superb save denied Brennan Johnson as the Imps were the better side in the first half.
Johnson was fouled in the box 13 minutes from time by Aristote Nsiala and Grant stroked home the resulting penalty.
Town ended the match with 10 men after former Lincoln midfielder Jon Nolan was sent off following a challenge on Harry Anderson in added time.
Peterborough took advantage of Ipswich's defeat to leapfrog them and Hull into second after coming from behind to beat the Tigers 2-1.
Second-half goals from Jonson Clarke-Harris and Siriki Dembele provided Darren Ferguson's in-form team with a fifth league win on the spin.
An away victory had looked unlikely once Mallik Wilks opened the scoring after 36 minutes.
Doncaster missed the chance to climb into the play-off zone after suffering a 2-1 home defeat to Crewe.
Josh Sims quickly cancelled out Charlie Kirk's opener for Crewe before Harry Pickering hit the winner.
Charlton made it three wins in a row after a routine 2-0 triumph at Northampton thanks to a Darren Pratley strike and an own goal from Jack Sowerby.
Will Keane hit the equaliser 21 minutes from the end as Wigan secured a 1-1 draw with Plymouth.
Paddy Madden and Ched Evans earned Fleetwood a 2-0 win at Gillingham and Oliver Rathbone and Alex Newby helped Rochdale to a 2-1 win at Shrewsbury.
Sullay Kaikai's goal saw Blackpool move out of the relegation zone following a 1-0 win over MK Dons and Burton and AFC Wimbledon played out a 1-1 draw.
Accrington's game with Bristol Rovers and the A420 derby between Oxford and Swindon were called off following a series of positive Covid-19 tests.
The results leave Sunderland sixth in the table, but with a game in hand on the five sides ahead of them.
Phil Parkinson's men travel to Rochdale on Tuesday night before another away day at Gillingham next Satuday.
