The pop singer, 54, has reprised her role of Charlene Robinson as the iconic soap comes to an end.
While UK viewers will have to wait till Friday night to see how the long-running show ends, Australian fans will watch the show today.
Posting a black-and-white throwback picture, Kylie said: "LOVERS… It’s almost time for the @neighbours finale!! Are you ready…??" She also tagged the Neighbours Instagram account.
In the snap, Kylie can be seen posing on the set of the soap opera outside the Lassiters hotel.
As she sat beside a small pond, she had her classic curly hairstyle and wore a polka dot skirt with a tank top and belt.
Many fans commented on the post to share their excitement for the upcoming episode, with Kylie's sister Dannii Minogue also revealing she will be tuning in.
One fan said: "Woo hoo can't wait."
Another wrote: "Agggh. So many memories Charlene," while a third commented: "Cant wait but so sad."
"Won’t miss this for the world," someone else said.
Other people said they weren't ready to say goodbye to the soap, with one fan commenting: "I will never be ready to see Neighbours end. Been watching since day one for 37 years and I feel sick at the thought of not getting my daily Neighbours fix."
Kylie will be joined by Jason Donovan who will return as her onscreen husband Scott Robinson.
Jason, 54, recently revealed the final episode of Neighbours was going to be "emotional" and said he understands expectations are high.
Appearing on The One Show, Jason said: "I'm not going to give too much away about the episode, but I can tell you it's going to happen, in what capacity, you are going to have to switch over to Channel 5 and watch.
Host Rylan Clark pressed Jason for more information and asked if the final scene would involve him and Kylie arriving on Ramsay Street and announcing: "We're home!"
Jason was clearly under strict orders not to reveal anything, but suggested Rylan "could be close".
He added: "It was emotional but it's filming and you know, it moves on. You'll have to wait and see."
Margot, 32, played Donna Freedman from 2008-2011, while Delta, 37, played aspiring singer Nina Tucker in 2002.
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*Neighbours: The Finale airs as a double episode special on Channel 5 on Friday, July 29 at 9pm.