We The Curious is entering a redundancy consultation with its staff due to the financial impact of the Covid-19 crisis.
The Bristol science centre said it has lost more than £1.9million in revenue this financial year since its enforced closure in March.
Following a significant reduction of visitor income, and with a lack of Government support packages for science centres, the venue said its financial situation is looking "extremely challenging".
A spokesperson for We The Curious, which is the region's only science centre, said "significant savings" urgently need to be made in every area - including 33 per cent of its annual salary bill.
The review process is now underway before a formal 30-day consultation process and restructure begins across all areas and levels of the charity which runs the site from September.
News of the restructure follows similar announcements from other cultural venues, science centres and museums across the UK.
We The Curious has said it will provide the fullest support to those employees whose jobs are at risk.
Donna Speed, Chief Executive for We The Curious, said: "This is a difficult and saddening time for our whole team, redundancies are our absolute last resort, and like many, we’ve tried everything to avoid being in this position.
"Since our enforced closure in March, we entered into the process with two key principles; to protect our staff and to protect our charity.
"Since then, our income has shrunk significantly, we’ve done everything that we can to remain resilient and constantly adapt but sadly the economic impact caused by the pandemic means that we simply can’t maintain our current staffing levels."
Donna said English science centres "seem to fall down the gaps in terms of support" as they are "neither a museum, a frontline crisis charity or an art gallery".
This is despite the fact they have "never been more needed by audiences," she added.
We the Curious relies on support from visitors, corporate partners and funders to run its social inclusion and education work and to deliver new exhibitions, such as Project What If, a funded project that was awarded £3m by the Inspiring Science Fund.
This groundbreaking new exhibition - which is the culmination of three years’ work - is due to launch when We The Curious reopens in November this year.
Donna added: "It's with heavy hearts that we’re now speaking with our teams, and starting the restructure process.
"Everyone has worked so hard over the past few years on Project What If, and we still plan to reopen in November with the new exhibition, but sadly our team will be in a different shape to what we’ve been working on until now."
