Joanna Cherry has called on Nicola Sturgeon's husband to resign from his role as chief executive of the SNP.
The MP for Edinburgh South West said that Peter Murrell should make way for a "neutral caretaker CEO".
Cherry has been a long-term critic of the gender reforms passed by MSPs at Holyrood.
Sturgeon stunned the political world yesterday when she announced her resignation as First Minister and SNP leader.
The SNP's finances have been under scrutiny since it was revealed last year that Murrell had loaned the party £107,000 of his own money.
A separate police investigation is also looking at crowdfunded donations the SNP received in 2017 from supporters.
A party spokesperson said Murrell had no intention of resigning.
Cherry is one of several Nationalists who have spoken out against the top two roles in the SNP being held by a married couple.
In a tweet, she said: "The SNP leadership and party management have been deeply bound together.
"I cannot see any circumstances in which Peter Murrell can continue as chief executive under a new leader who must be free to choose a successor. Meantime we need a neutral caretaker CEO.”
Cherry was appointed to the SNP frontbench in Westminster after her election in 2015 but was removed in 2021.
The KC said that she had been "sacked" despite "hard work, results and a strong reputation".
An SNP spokesperson said that she was not removed because over her views on gender.
The spokesperson said in a statement: "Joanna Cherry was removed from the front bench because of unacceptable behaviour, which did not meet the standards expected of a front bench spokesperson – not because of the views she holds."
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