Jarrell Miller has denied deliberately cheating after failing a drugs test ahead of his fight against Anthony Joshua.
Miller tested positive for a banned substance and has been denied a licence in New York.
Joshua will now need to find a new opponent for his fight at Madison Square Garden on June 1.
But Miller insists he is innocent.
He said: "I want to thank all of my supporters who have stood by me since day one and especially during this extremely difficult time.
"I have not been tuned in to social media and any other media outlets to focus and channel all of my energies into working hard with no distractions.
Anthony Joshua vs Jarrell Miller officially off as American is refused licence
"I am absolutely devastated upon hearing the news my boxing license has been revoked in NY State and I will be vigorously appealing this decision. I have NEVER knowingly taken any banned substance and when I found out the news last night I was totally shocked.
"My team and I stand for integrity, decency & honesty and together we will stand to fight this with everything we have!
"This was a voluntarily test that I was very happy to do and these results came just one week after another voluntarily test that I had taken which was completely clean. I refuse to just lie down and let my dream be taken away from me when I know in my heart that I’ve done nothing wrong.
"15 years of hard work. I’m WARRIOR .. I don’t need a banned substance.
"Remember don’t believe everything you hear and see. The facts will prevail and I shall be vindicate. I don’t have anything to hide and the truth will make itself known."
