Japan Airlines has said it is swapping "ladies and gentlemen" for gender-neutral greetings.
It follows other global airlines who recently adopted more inclusive language for passengers.
Announcements at airports and on flights operated by the Japanese carrier will use the new forms of address from October 1, the airline said.
"Attention all passengers" and "Good morning everyone" will be among the terms adopted, according to local media.
Several airlines around the world have made a similar change in recognition of non-binary and transgender customers.
Air Canada and European low-cost carrier EasyJet said last year they would drop "ladies and gentlemen".
Japan Airlines (JAL) spokesman Mark Morimoto said: "We aspire to be a company where we can create a positive atmosphere and treat everyone, including our customers, with respect.
"We have committed to not discriminate based on gender, age, nationality, race, ethnicity, religion, disability, sexual orientation, gender identity or other personal attributes."
The announcement comes as gender-equality advocates say corporate support for LGBT+ rights is growing in socially-conservative Japan, where same-sex marriage remains illegal and being openly gay seen as taboo.
In April, a Japanese charity that launched a scheme offering digital partnership certificates - allowing same-sex couples to tap into the same staff benefits as heterosexual couples - won the backing of businesses from banks to insurers.
About a third of Japanese companies have measures in place to support gay couples, according to campaign group Nijiiro Diversity.
But activists say discrimination persists, and even though about two dozen cities, towns and wards issue same-sex partnership certificates to gay couples, they lack legal standing.
In March, JAL announced it would allow female flight attendants to wear trousers and ditch their high heels at work, following a feminist campaign.
