Rome's underground system has been evacuated after two more earthquakes were felt in the capital.
The European-Mediterranean Seismological Centre (EMSC) measured the earthquake at magnitude 5.7, larger than the 5.3 event that struck Italy earlier on Wednesday morning.
The earthquake, recorded at 11.14am local time (10.14am GMT), was followed just 10 minutes later by another tremor measured at magnitude 5.3. by the US Geological Survey.
There were no immediate reports of casualties or serious damage. Antonio Tajani, the Italian president of the European Parliament, said tremors were “felt as far as Rome (but it) appears there are no victims.”
All three events had epicentres recorded in Central Italy, in regions devastated by the August earthquake that killed almost 300 people.
Seismologists say previous quakes have stressed the Laga Fault, which is rupturing.
The August earthquake was followed by at least two more deadly earthquakes in Umbria and Marche in October, both measuring over six on the Richter scale.
Italy's Appenine mountains run along a fault line between two tectonic plates, causing regular seismological events.