
Vampire Survivors now has a Balatro collaboration in the form of the free Ante Chamber DLC. The 1.14 game update introduced this addition seemingly out of nowhere with four new characters, four extra weapons, and a Special stage.
While the Ante Chamber DLC is free, you need to find and buy the Joker Disk to unlock and play it. You can buy it for 50,000 Gold Coins from Xanthia in the Moongolow Bonus Stage. For casual Vampire Survivors players or those returning to the game after a long time, that means you might need some extra steps to unlock the Balatro DLC.

If Moongolow isn't in your list of stages, you need to first unlock Hyper Mode for four out of the five normal stages (Mad Forest, Invalid Library, Dairy Plant, Gallo Tower, or Capella Magna). This involves defeating the mini-boss that spawns at the 25-minute mark in each stage. Once you do it, you'll see Moongolow in the Stage Selection window under Il Molise with an exclamation mark next to it.
Enter Moongolow and move towards Xanthia. You can see their location on the map marked by a mask with a blue flame around it, as shown in the image below. Once you approach them, a window will pop up to let you buy the Joker Disk for 50,000 Gold Coins.

After you acquire the Joker Disk, you can conclude your run to see the Ante Chamber unlocked after you leave it. Unlike paid DLCs, Ante Chamber is a Special Stage, not an Adventure. You will find it in the Stage Selection menu from the standard Start button on the main screen of Vampire Survivors.
The Ante Chamber is themed after Balatro. Your character will walk over the card game's interface, with buttons serving as walls and barriers, and cards serving as bridges. I highly recommend you work towards the question mark on the map in your first run so you can unlock Jimbo, one of the four new characters from the DLC.
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