Holly Willoughby screamed she was "going to wet herself" as she struggled to contain her laughter on This Morning, alongside co-host Phillip Schofield.
The pair got the giggles after being set-up by producers, catching them off guard with the autocue.
Holly and Phil were reading out the teaser for the following episode, to give viewers an insight into what would be on the show.
But the unexpected autocue message that flashed up for Phil to read left them both confused over if it was even real.
Suddenly, in clips played out during Tuesday's show, the hosts began laughing with actual tears in their eyes.
Phillip had learned they were going to be taught how to milk a horse on the show - something Josie Gibson ended up taking part in on Tuesday.
Barely able to string a sentence together, the hosts were so stunned that they could not continue and had to film the short teaser again.
What only airs for around 10 seconds took them eight minutes to film, because they couldn't stop laughing over the looming segment.
Holly kept stumbling on her words knowing what was coming, while Phil was heard asking the backstage crew if it was actually happening.
After several attempts they got through it, but not before a series of giggles and Holly almost wetting herself.
Phillip shouted as he fought back laughter: "What the hell was that?! Is that real, or did you just do that to blow me up? Is that real?"
The pair kept wheezing hilariously, as Phil said: "It's gonna be quite hard for me to say it."
Holly shouted out: "I'm gonna wet myself, I'm actually gonna wet myself. It's coming out of my eyes now.
"My nose is running, it's coming out of everywhere... now my ears are ringing."
This Morning airs weekdays at 10am on ITV.
