Mike Ashley has already axed eight Jack Wills stores since taking the company over earlier this month.
The billionaire acquired the "preppy" lifestyle brand via a pre-pack administration deal worth £12.8 million on 5 August.
And now eight stores have been closed out of the 100 it took over under the deal.
The store closures are in Marlborough, Derby, Reigate, Rock, Tunbridge Wells, Durham, Kingston and St Albans.
On Thursday, Sports Direct said it would endeavour to redeploy the staff affected elsewhere within Jack Wills.
Earlier this week it emerged that Sports Direct is negotiating with Jack Wills’ landlords to accept rent-free tenancy agreements across some of its worst-performing stores.
Speaking on the sale, Jack Wills chief executive Suzanne Harlow said: "For the past year, we have been focused on improving the Jack Wills proposition and the group's financial performance.
"Despite significant progress, the challenging trading environment led us to conclude that the company's long-term future would be best served as part of a larger group and Sports Direct will enable us to do this."
Jack Wills continues to occupy stores on license from its administrators until its new owner can negotiate fresh lease agreements.
