France has scrapped talks with Home Secretary Priti Patel in an explosive escalation of tensions over the handling of deaths in the Channel.
French Interior Minister Gerald Darmanin withdrew an invitation to a critical meeting on the crisis after branding Boris Johnson's demands "unacceptable".
The meeting, attended by other European ministers, will go ahead on Sunday.
The Prime Minister enraged France by publishing a letter on Twitter with five demands, including sending Brits to do joint border patrols on the French coast.
In an extraordinary diplomatic spat, President Emmanuel Macron accused the UK of not being "serious" over tackling the crisis.
But Downing Street said the PM did not regret posting his letter on Twitter.
It comes after 17 men, seven women and three teenagers died in the Channel on Wednesday as they attempted to make the perilous crossing to the UK.
French media reported that the Interior Ministry statement said: "We consider the British Prime Minister's public letter unacceptable and contrary to our discussions between counterparts.
"Therefore, Priti Patel is no longer invited on Sunday to the inter-ministerial meeting whose format will be: France, Belgium, Netherlands, Germany and European Commission."
Mr Macron issued a stern rebuke to the UK at a press conference in Rome on Friday.
"I'm surprised when things are not done seriously, we don't communicate between leaders via tweets or published letters, we are not whistle-blowers", he said.
A French Government spokesman said the PM's letter "doesn't correspond at all" with discussions Mr Johnson and Mr Macron had on Wednesday.
"We are sick of double-speak," he said.
Downing Street insisted the PM did not regret posting his letter on Twitter.
The Prime Minister’s spokesman said: “As the PM has said himself, both the PM and President Macron have a shared recognition of the urgency of the situation we are both facing.
“You will see from the tone of the letter, this is about deepening our existing cooperation and building on the work that’s already being done between our two countries.”
No10 hit back at Mr Macron's claims, saying: “This is an issue that we are taking extremely seriously.”
Earlier, Transport Secretary Grant Shapps urged France to reconsider, saying that "neither nation can tackle this alone".
He told LBC: "I very much hope the French will reconsider. Talking is obviously the way forward."
In his letter, the Prime Minister said both two countries needed to "go further and faster together" to deal with the crisis.
He set out proposals for British border officials to begin patrols on the beaches of northern France as early as next week - something Paris has long resisted.
Ms Patel had been due to visit Calais on Sunday for talks with Mr Darmanin and counterparts from Belgium, the Netherlands and Germany.
Mr Johnson had said he was ready to upgrade this meeting to a full summit of the countries concerned.
Liberal Democrat Home Affairs Spokesperson Alistair Carmichael accused the PM of "chasing headlines ahead of finding solutions".
He said: "At a time we need competence and strong leadership to tackle this crisis, the prime minister has shown he is not up to the task.
“Neither Boris Johnson’s tweets nor the French Government cancelling talks will solve this problem. We need grown-up leadership on both sides of the Channel to tackle this crisis and put saving lives over political posturing."
The Home Office declined to comment.
