Major plans to transform industrial land overlooking the Feeder Canal will go before Bristol City Council in two weeks.
Proposals for the area around Silverthorne Lane, east of Temple Island, include hundreds of homes, offices, shops, student accommodation and a secondary school.
Councillors will discuss the development, brought by Feeder Estates - a partnership managed by Square Bay - during a virtual meeting on May 27.
However, scores of residents have complained about the proposals, with many voicing concerns it could lead to Motion nightclub's closure.
Alex MacDermott wrote to the council saying: "The plans as they stand can not go ahead.
"Motion, which has been a core part of Bristol's nightlife for over a decade is an instrumental part of the Bristol music scene, which has helped to create Bristol's culture and made it a popular attraction to millions of visitors and tourist each year is under extreme threat of closure."
While Megan Glaister said: "The Bristol nightlife is what makes it one of my favourite places to visit, and getting rid of Motion would mean you are getting rid of many people's favourite club and one of the biggest highlights of the city."
What are the plans?
Land on the half-kilometre stretch of road had been home to warehouses and businesses which were due to leave in the summer.
The development area is split into six sites along Silverthorne Lane - from the Avon Street junction near nightclub Motion and the railway bridge.
Bosses at the the Department for Education chose the site to deliver a new 1,600 secondary school and sixth form to address a looming shortage of places in the city.
The school, Oasis Academy Temple Quarter, will be operated by Oasis Community Learning who already operate eight schools across the city.
The first sites, opposite Motion, have been designated as a mixture of office and commercial space being delivered in partnership with the University of Bristol.
Next door - plots two and three - will be the residential aspect. Award-winning Bristol-based housebuilder, Studio HIVE, in partnership with Atlas Land Ltd, will be delivering approximately 371 new homes to the site, 20 per cent of which will be affordable.
Behind the residential aspect - next to Silverthorne Lane - will be more office and commercial space also being delivered by Studio HIVE.
Just along from the residential plot is the secondary school, which takes up the largest amount of development land.
The final site, nearest to St Philip’s Causeway, has been designated for student accommodation - with around 693 student beds planned.
