Jordan Pickford will be sidelined until at least after the international break because of injury.
The Everton goalkeeper was forced off before half-time during Saturday's game with Burnley after hurting his oblique abdominal muscle.
Pickford, who was replaced by Joao Virginia in goal, underwent a scan on Monday to determine the full extent of the problem.
Surgery is seen as unlikely at this stage but Pickford will miss England's three World Cup qualifying matches later this month and so has been ruled out of Saturday's FA Cup quarter-final tie with Manchester City.
Robin Olsen has not yet given up hope of being fit for the game at Goodison.
A club statement read: "Everton goalkeeper Jordan Pickford will miss England’s trio of World Cup qualifying fixtures this month after sustaining an injury to his oblique abdominal muscle.
"Pickford, 27, was forced off after 43 minutes during Saturday’s Premier League meeting with Burnley and underwent a scan on Monday.
"He is ruled out of England’s games against San Marino, Albania and Poland, as manager Gareth Southgate’s team begin their bid to reach the 2022 World Cup finals in Qatar.
"Pickford, who has made 154 Everton appearances since joining from Sunderland in July 2017, will remain under the care of the club's medical staff at USM Finch Farm during the forthcoming international break."
