The Scottish Tory leader is seeking to strike up talks with the leaders of other pro-UK parties after he stated that the launch of Alex Salmond's new Alba Party was an attempt to "game the system" in favour of Scottish independence.
Douglas Ross is calling on Scottish Labour and the Scottish Liberal Democrats to form a "pro-UK anti referendum coalition" if possible in a bid to stop pro-independence parties winning a majority at Holyrood.
He added that the launch of the new Alba Party in an attempt to win seats on Holyrood's regional lists could create a "supermajority" in favour of Scotland quitting the UK".
Ross stated that "if the numbers allow", the three 'anti-referendum' parties should work together to get the focus of Scottish politics "back on to the issues that matter to the people", which, according to him is not a "damaging, divisive, destructive referendum campaign".
Adding that the pro-UK parties could even agree to only run one candidate in constituency seats, moving towards pushing whichever candidate has the best chance of defeating the SNP, he said that "nothing is off the table".
The Conservative MP has already written to Anas Sarwar and Willie Rennie, saying: "We've got to sit down as the leaders of the three main pro-UK parties in Scotland, as we did in 2014, to look to work together to stop them."
He insisted that action was needed because Friday's launch of the Alba Party - which will see Mr Salmond run as a candidate for the North East region - had "changed the nature of this campaign".
Ross told Sarwar and Rennie: "We've got to address this. If we don't the Nationalists will dominate our politics."
He wants the other two pro-UK parties to agree to sign up to pledge committing them to voting against any proposal for a second Scottish independence referendum, and making clear they will not form a coalition with any of the pro-independence parties.
The pledge also states that "if the numbers allow", the three main pro-UK parties would form an "anti-referendum coalition" in the Scottish Parliament.
Speaking about the plans, Ross said: "It is clearer than ever before that the dividing line in this election is now over another independence referendum.
"I think that is an extremely sad state of affairs when we should be focused on our recovery and rebuilding after Covid-19 and sorting out 14 years of failure from the SNP Scottish government.
"But there is no doubt that the Nationalists, whether they be Nicola Sturgeon's Nationalists or Alex Salmond's Nationalists, want to take us through another divisive independence referendum.
"That means we can't focus on our recovery and rebuilding.
"That's why I am asking for all the pro-UK parties to do what we did in 2014 and come together, put political differences aside, and stop this drive towards another independence referendum."
Scottish Liberal Democrat campaign chair Alistair Carmichael however insisted that the Tory leader's politics were "far too dark and divisive".
Carmichael said: "Lib Dems will work with others to deliver a constructive and ambitious plan for recovery, but Douglas Ross's politics are far too dark and divisive.
"We will focus on winning seats and ensuring that the next government is focused on putting the recovery first, not independence.
"As a football referee Douglas Ross has a knack for uniting the fans of opposing teams. As a party leader he seems to do the exact opposite."
Anas Sarwar said Mr Ross "needs to grow up".
The Scottish Labour leader added: "He needs to recognise that we are in the middle of a pandemic.
"He needs to recognise that this election is not some kind of game, it's not some kind of battle, it's not about party politics, it's not about individual politicians fighting with each other - it's about focusing on a national recovery."
Ross later said it was "incredibly disappointing that other parties won't even come to the table to discuss how we can work together".
