Scots travelling from the Canary Islands must now quarantine.
The UK Transport Secretary, Grant Shapps, announced that the holiday hotspot has been removed from the UK travel corridor list.
Anyone arriving in the UK from the Spanish islands has to self-isolate.
The rule comes into force at 4am on Saturday.
It comes after droves of holidaymakers booked to visit the Canaries when they were added to the travel corridor list in October.
The removal comes after a surge in weekly cases and positive tests of deadly Covid-19.
Travellers to mainland Spain already have to isolate on their return to the UK.
Paul Charles, chief executive of travel consultancy The PC Agency, said: "It's utterly devastating news for the thousands of British travellers who booked to go to the Canaries for Christmas and New Year.
"It's also a body blow for travel firms who'd seen an uplift in bookings for the winter after the Canaries were added to the travel corridor list.
"It now means thousands of refunds and lost bookings for a sector that needed the Canaries to help them recover."
Botswana and Saudi Arabia have been added to the travel corridors list.
Most people returning to the UK from destinations not on the list must self-isolate for 14 days.
This quarantine period can be shortened for people arriving in England from December 15 if they take a negative coronavirus test at least five days after they land.
England has also introduced a quarantine exemption for certain categories of travellers, including people making high-value business trips, sports stars and performing arts professionals.
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