Boris Johnson’s model-turned-tech businesswoman friend questioned whether unfaithful husbands deserve sympathy in a documentary about a notorious infidelity website.
The Mirror can reveal Jennifer Arcuri appears in a film about Ashley Madison, a service used by married men and women to arrange illicit sexual liaisons online.
Ms Arcuri discusses the 2015 hacking of the site - which exposed hundreds of affairs - in the film Ashley Madison: Sex, Lies and Cyber Attacks.
“Is it OK to cheat on your wife?” she said. “Because you can do it behind closed doors on this website.
“And then what happens when that is exposed? Are we all supposed to feel sorry for you?”
She is also shown teaching a class on lockpicking at her startup business Hacker House, which aimed to teach cyber security skills using computer hacking techniques.
The documentary also features Lauri Love, whom the Mirror revealed today had worked at Hacker House while he was fighting extradition to the US, accused of hacking into the FBI.
The London Assembly wrote to Mr Johnson, demanding an explanation of his links with Ms Arcuri within 14 days.
The letter, written by chair of the London Assembly oversight committee, Len Duvall, demands Mr Johnson provide information about his “personal, social and professional” links to Ms Arcuri.
The PM has refused to directly comment on the conflict of interest allegations - or on the nature of his relationship with Ms Arcuri.
But speaking about his time as mayor, he said: “Everything was done with complete propriety.”
And Ms Arcuri was said to have denied having a relationship with Mr Johnson, according to the Daily Mail.
His daytime visits to her flat were, she’s said to have claimed, for ‘technology lessons’.
It follows claims that while in the role between 2008 and 2016, Mr Johnson failed to declare a potential conflict of interest in relation to the US businesswoman.
Her company Innotech got £11,500 in sponsorship from the mayor’s promotional agency during this period.

And it is alleged Ms Arcuri received special treatment to attend three foreign trade missions with the then mayor in the space of a year.
Ms Arcuri said in a statement: “Any grants received by my companies and any trade mission I joined were purely in respect of my role as a legitimate businesswoman.”
Ms Arcuri, 34, is understood to have begun her close friendship with Mr Johnson, 55, in 2012 when she volunteered on his re-election campaign as mayor.