Boris Johnson had indicated that he would not be prepared to "take the knee" in support of the Black Lives Matter movement.
The Prime Minister seemingly suggested that he is not prepared to follow the example of Labour leader Sir Keir Starmer - who was photographed in the symbolic act of support - as he believes in "substance" not "gestures".
He went on to say that he doesn't want people to be "bullied" into things they do not want to do.
Taking the knee was first made a gesture in the National Football League (NFL) in 2016 after San Francisco 49ers quarterback, Colin Kaepernick, sat and later knelt during the anthem.
It has been brought back into more prominence in recent months following the death of George Floyd in America.
Thousands have made the gesture at demonstrations across the world as a sign of solidarity with the Black Lives Matter movement.
The first games of the Premier League restart saw players take the knee before the matches began.
However, speaking on an LBC radio phone-in, Mr Johnson expressed concern that some police officers had been pressurised into doing so during protests in London following the death of George Floyd in the United States.
"I don't want people to be bullied into doing things they don’t necessarily want to do," he said.
"If you think of what happened with those police officers standing at the Cenotaph, where they were being insulted in quite aggressive terms by some members of the crowd and told to take the knee.
"Some of them did and it was very difficult then for the others who didn't. That's my position.
"I think it is very, very important that you don’t do things that make life difficult or embarrassing."
His comments came after Foreign Secretary Dominic Raab was criticised last month for saying he would only take the knee for "the Queen and the missus when I asked her to marry me".
The Foreign Secretary sparked outrage after he stated that he feels it's a 'symbol of subjugation and subordination' and thinks the gesture has come from popular TV show Game of Thrones.
Mr Johnson has said he believes in practical action to tackle prejudice and discrimination.
"I don't believe in gestures, I believe in substance. I believe in doing things that make a practical difference. Of course there are injustices that we need to rectify.
"There is prejudice out there. We need to fight it," he said.
The Prime Minister also indicated during the conversation on LBC that he wants to see more black people at the top of government.
