Civil servants were evacuated from St Andrews House in central Edinburgh following the discovery of a suspicious item.
A bomb disposal team was called to the Scottish Government's premises on Regent Road, who later found the item was "innocent in nature."
Police Scotland said the building was evacuated as a precaution and there was no risk to the public.
It follows a similar incident on Holyrood Road on Tuesday, which saw a controlled explosion carried out.
Superintendent Mark Rennie said: "Police were called to St Andrew's House in Edinburgh after a suspicious item was found within the building.
"As a precautionary measure, in accordance with procedures, staff were evacuated from the building and a cordon implemented.
"Following examination of the item by EOD (Explosive Ordnance Disposal) it was confirmed that the item posed no threat to the public and was innocent in nature."
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