The Market Inn, in Auchinleck, has closed its doors after a positive test of coronavirus was linked to the pub.
NHS Ayrshire & Arran Test and Protect team say they are carrying out Covid-19 contact tracing.
Those who have been identified as close contacts through this process have already been contacted directly by the Test and Protect Team and advised to self-isolate for 14 days.
However, anyone who visited the pub between Sunday, September 6 and Friday, September 18 are asked to "be extra vigilant" and, if they develop any of the symptoms of a fever, new persistent cough or loss of taste or smell they "need to self-isolate immediately."
Lynne McNiven and Joy Tomlinson, Directors of Public Health, said: "NHS Ayrshire & Arran Test and Protect team is carrying out Covid-19 contact tracing related to the Market Inn in Auchinleck.
"Those who have been identified as close contacts through this process have already been contacted directly by the Test and Protect Team and advised to self-isolate for 14 days.
"The Market Inn has physical distancing and hygiene processes in place to minimise the risk of viral spread and to support Test and Protect. The risk to the public is considered low.
"We are however asking customers who have been in the pub between 6 and 18 September to be extra vigilant and, if they develop any of the symptoms of a fever, new persistent cough or loss of taste or smell they need to self-isolate immediately and contact the Health Protection Team for further advice on 01292 885858, select Option 1.
"All relevant Public Health measures are in place and our Public Health Team is working with East Ayrshire Environmental Health Officers to manage and monitor the situation.
"The Market Inn is currently closed until the contact tracing is complete.
"To respect and maintain patient confidentiality it is not possible to release any further information at this time."
