Nina Kennedy has been unable to match the performance of her training partner Kurtis Marschall, who came out of isolation to book a spot in an Olympic pole vault final.
The women's qualifying round was interrupted for more than an hour by heavy rain in Tokyo on Monday night.
When the competition resumed, Kennedy had two remaining attempts at 4.55m - but was unsuccessful with both of them.
The West Australian vaulter holds the Australian record of 4.82m, but came into the Tokyo Games under an injury cloud.
Kennedy's preparation was further compromised when she, Marschall and Australian coach Paul Burgess were ordered into isolation on the eve of the Games after being ruled to be contacts of leading US vaulter Sam Kendricks, who tested positive to COVID-19.
The three Australians subsequently returned multiple negative results.
Fellow Australian women's vaulter Elizaveta Parnova was also eliminated in the qualifying round after missing three times at 4.40m.
Marschall will contest the men's vault final on Tuesday evening.
