Arsenal's Vinai Venkatesham, Liverpool's Tom Werner and Manchester United's Ed Woodward are among the five senior officials to stand down from their roles with the Premier League.
The mass removal follows the doomed European Super League venture, with the five chiefs all belonging to clubs who signed up to the controversial breakaway competition.
Red Devils executive vice-chairman Woodward and Liverpool chairman Werner no longer work with the Club Broadcast Advisory Group.
While Chelsea chairman Bruce Buckave has left his post with the Premier League's Audit and Remuneration Committee.
Arsenal and Manchester City's chief executive officers Venkatesham and Ferran Soriano, meanwhile, no longer work with the top-flight's Club Strategic Advisory Group.
The quintet was effectively forced out by their counterparts at the remaining 14 Premier League clubs who had nothing to do with the European Super League venture.
The group campaigned hard to have the five men removed from influential positions within the top-flight's committees.
Premier League chief executive Richard Masters also wrote to the five officials and asked them to reconsider their positions within the organisation following the actions of two weeks ago.
The 14 other Premier League clubs are keen for the so-called big six to be punished for signing up to the European Super League.
But the Premier League is currently looking into the circumstances surrounding the competition and still deciding whether any of the organisation's rules were broken.
