The 24-hour stoppage by drivers from ScotRail caused disruption for tens of thousands of rail users across Scotland.
The strike was the second in the past week and will be followed by two further strikes later in the month.
The 24-hour walkout by Arriva Trains Northern conductors will begin on March 28, followed by two 48-hour stoppages on April 5-6 and May 3-4.
The RMT, which is seeking a substantial increase in the pay of conductors, has already held several days of industrial action in recent weeks which has crippled Arriva's train services across northern England.
Today's ScotRail strike went ahead after Aslef and RMT leaders failed to settle their differences with management during talks held under the chairmanship of the conciliation service, Acas, yesterday.
The unions are demanding a substantial pay rise for ScotRail drivers, who they say are among the worst paid in the country.
The AA said traffic was slightly heavier on Scottish roads as a result of the strike.
A spokesman said approaches to Edinburgh on the M8 and M90 were busier than normal during the morning rush hour.
However, he said rain and high winds were causing bigger problems than the volume of traffic.
ScotRail was running a number of bus services into Glasgow from Airdrie, Lenzie, Neilston, Troon, Kilmaurs, West Kilbride and Ardrossan harbour.
Passengers were advised to call 0870 608 2608 for timetable information.
GNER services to and from London King's Cross, Inverness, Aberdeen, Glasgow Central and Edinburgh were unaffected.
