A group of people protesting against Covid-19 measures gathered outside the family home of Dr Tony Holohan on Sunday.
The small gathering saw protesters rallying against the coronavirus vaccine and masks.
Some people carried placards questioning the safety of the jab, while others had signs stating, 'Leave our kids alone'.
The gathering was streamed live on social media from outside the Chief Medical Officer's Dublin home where he lives with his two children.
Gardai confirmed they had attended a protest in Dublin 6 on Sunday afternoon but did not refer to the CMO.
A spokesperson said: "Gardaí were alerted to a number of protesters outside a residence in Dublin 6 this afternoon Sunday, 3rd October, 2021, at approximately 1pm.
“Gardaí attended and the protest ended without incident at 2.30pm.”
It comes after protestors held a rally outside the home of Tanaiste Leo Varadkar and his partner twice last month.
On the first occasion, gardai were called to break up a protest on September 19 outside Mr Varadkar's house in Dublin.
Protestors gathered outside the house the Tanaiste shares with his partner Matt Barrett and proceeded to make unfounded allegations about his sexual orientation.
In a video posted online, people among the small group gathered outside his Dublin home were heard shouting homophobic remarks and calling Mr Varadkar a "treasonous snake".
Gardai were also alerted to this protest and attended the scene.
Protestors gathered outside the residence for a second time a week later with gardai called to the scene again.
Video footage circulating online showed a number of people standing outside the politician's home with some carrying signs.
'Freedom of speech, freedom of choice' was among some of the messages displayed on the placards.
