Prime Minister Anthony Albanese stands by comments he made about human rights abuses in Iran following reports the Australia ambassador had been summoned.
But it remains unclear the specific comments Iran has taken issue with or when they were made.
Australia's ambassador Lyndall Sachs was summoned by Tehran on Wednesday.
Nationwide protests began over two months ago in Iran following the death of young woman Mahsa Amini in the custody of the morality police.
Since then, Tehran has summoned several foreign ambassadors over comments regarding protests made by their officials.
"I stand by all of the comments made about the human rights abuses that have occurred in Iran, arising from Mahsa Amini's murder and then the subsequent actions that have occurred," Mr Albanese told reporters in Bali.
"We will stand up for human rights (and) we will stand up for Australia's position consistent with our values."
Asked if Iran was referring to comments he made at the G20 leaders' summit, Mr Albanese shook his head.
In a statement on Wednesday, Iran's foreign ministry spokesperson Nasser Kanaani said it "seems that the prime minister of Australia has taken a wrong approach" based on "false information".
With Reuters
